Proliferative responses of β₂GPI-specific CD4⁺ T-cell clones to 15 synthetic peptides covering the entire domain V.

The β₂GPI-specific CD4⁺ T-cell clones OM2 and OM8 were cultured with autologous APCs for 3 days in medium alone or in medium supplemented with individual synthetic peptides (5 μg/mL), and peptide-induced T-cell proliferation was measured by³H-thymidine incorporation. GP-F, GP1, GP2, GP3, and MalBP (10 μg/mL) were also used as antigens for T-cell proliferation. Significant T-cell proliferation to domain V peptide or recombinant β₂GPI in comparison with the respective controls is shown as an asterisk. Similar results were obtained in 4 independent experiments.