Peptide-induced proliferative responses of β2GPI-specific CD4+ T-cell clones incubated with L-cell transfectants expressing human HLA class II molecules.
The β2GPI-specific CD4+ T-cell clones OM2 and OM8 were incubated with a series of L-cell transfectants in the presence or absence of p276 to 290 (10 μg/mL) and p247 to 261 (5 μg/mL), respectively. The peptide-induced T-cell proliferation was measured by 3H-thymidine incorporation. In some experiments, T-cell clones were cultured with peptide-pulsed L cells in the presence of anti-HLA-DR or anti-HLA-DQ mAb (1 μg/mL). Significant T-cell proliferation in comparison with the control culture without antigenic peptide is shown as an asterisk. The results were similar in 2 independent experiments.
