In vitro production of anti-β₂GPI antibodies in cultures of β₂GPI-specific CD4⁺T-cell clones and autologous B cells stimulated with GP-F.

Peripheral blood B cells were cultured with β₂GPI-specific CD4⁺ T-cell clones in the presence or absence of antigen (GP-F, MBP, or TT; 10 μg/mL) for 10 days. Anti-β₂GPI antibody levels in undiluted culture supernatants were measured by ELISA. Significant anti-β₂GPI antibody production in culture with antigen compared with the control culture without antigen is shown as an asterisk. A representative result of 3 independent experiments is shown.