Fig. 6.
Effects of anti-HLA class II, anti-CD40L, anti-IL-6, and anti-IFN-γ mAbs on in vitro anti-β2GPI antibody production.
Autologous peripheral blood B cells were cultured with β2GPI-specific CD4+ T-cell clones (OM2 and KS3) and GP-F (10 μg/mL) in the presence of anti-HLA-DR, anti-HLA-DQ, anti-HLA-DP, anti-CD40L (1 μg/mL), anti-IL-6, or anti-IFN-γ (25 μg/mL unless indicated otherwise) mAbs for 10 days. Anti-β2GPI antibody levels in undiluted culture supernatants were measured by ELISA. Significant inhibition of anti-β2GPI antibody production by anti-HLA class II or anticytokine mAbs in comparison with the control culture with isotype control mAb is shown as an asterisk. Similar results were obtained in 3 independent experiments.
