Fig. 3.
Effect of cathepsin G and neutrophil elastase on binding of P-selectin to neutrophils.
Specific binding of 125I-labeled P-selectin (1 μg/mL, final concentration) to neutrophils (2 × 107/mL, final concentration) pretreated with cathepsin G (A and B) or neutrophil elastase (C and D). For the dose-response curves (A and C), the digestion time was 30 minutes at 22°C. For the time course (B and D), the final concentration of the enzymes was 20 μg/mL. Data are representative of 3 separate experiments.
