Fig. 4.
Fig. 4. Digestion of KG1a lysates. / Time course for the digestion of KG1a lysates with cathepsin G (left panels) or neutrophil elastase (right panels) at a final concentration of 20 μg/mL. Upper panels were immunoblotted with rabbit anti–PSGL-1 N-terminal peptide IgG, and lower panels were immunoblotted with the anti–PSGL-1 monoclonal antibody PL-2. Blots were visualized using the ECL method. Prestained molecular weight markers are myosin (250 kd) and bovine serum albumin (98 kd).

Digestion of KG1a lysates.

Time course for the digestion of KG1a lysates with cathepsin G (left panels) or neutrophil elastase (right panels) at a final concentration of 20 μg/mL. Upper panels were immunoblotted with rabbit anti–PSGL-1 N-terminal peptide IgG, and lower panels were immunoblotted with the anti–PSGL-1 monoclonal antibody PL-2. Blots were visualized using the ECL method. Prestained molecular weight markers are myosin (250 kd) and bovine serum albumin (98 kd).

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