Effects of plate-bound and soluble CD30-agonistic antibodies on Karpas 299 cells.

Karpas 299 cells were treated for 20 hours either with plate-bound CD30 antibodies M44 and M67 (provided by Immunex Corporation), as indicated, exactly as described previously,1-3 or with the soluble antibodies at the indicated concentrations. Cell viabilities were evaluated by propidium iodide exclusion and flow cytometry, and normalized to the IgG1 isotype control as described previously.1-3 The experiment was performed in triplicate, and standard deviations are shown.