Fig. 5.
Down-regulation of p27kip1 is mandatory for induction of bcl-2 expression and promotion of viability by IL-7 in T-ALL cells.
(A) T-ALL cells were cultured for 72 hours under the indicated conditions, and the percentage of viable cells was examined by propidium iodide staining followed by FACS analysis as described in Materials and methods. (B) T-ALL cells isolated from the indicated culture conditions were examined for viability as above and for expression of bcl-2 protein levels by intracellular staining and by FACS analysis, as described in Materials and methods.
