HTLV-I proviral load in purified T-cell subsets.

CD4⁺ T cells or CD8⁺ T cells were negatively selected from PBMCs. The purity of isolated populations is shown in Table 1. HTLV-I proviral load was measured using quantitative PCR for HTLV-I pX region and human β-actin. The gray bars indicate HTLV-I proviral load per 100 PBMCs, the striped bars indicate proviral load per 100 purified CD4⁺ T cells, and the black bars indicate proviral load per 100 purified CD8⁺ T cells.