The comet tail shape, intensity, and length differed according to whether the cells were treated in suspension or adhered to FN.

The comet moment is a function of the distance and intensity of DNA from the center of the comet head. (A) Results with 0.1% DMSO. (B) Results with 0.5 μM etoposide. (C) Results with 5.0 μM etoposide. (D) Results with 50 μM etoposide. (D-E) Cells adhered to FN for 2 hours showed a significant (P < .01) reduction in mitoxantrone- and etoposide-induced DNA double-strand breaks. The neutral comet assay was used to compare drug-induced DNA double-strand breaks in cells adhered to FN and those cultured in suspension. Cells were exposed to various concentrations of either mitoxantrone (E) or etoposide (F) for 1 hour. The comet moment was then calculated. Fifty images were captured for each dose, and 3 independent experiments were done. The graph represents the mean values and 95% CIs from 3 independent experiments.