Cellular adhesion to FN altered the nuclear distribution of topo IIβ.

After 2 hours of cellular adhesion to FN, cells were fixed and stained for either topo IIα or topo IIβ. One hundred cells were analyzed for pixel density of the cytoplasm and nucleus. The nuclear-to-cytoplasmic ratio for topo IIα for cells grown in suspension was 7.56 ± 0.51, whereas that for FN-adhered cells was 5.39 ± 0.37. For topo IIβ, the nuclear-to-cytoplasmic ratio was 6.01 ± 1.58 for cells grown in suspension and 4.87 ± 0.33 for cells adhered to FN.