Fig. 7.
Fig. 7. Adhesion of cells to FN increased the amount of topo IIβ bound to the nucleus after a 1.0-M NaCl extract. / Nuclei isolated from FN-adhered cells or cells in suspension were sequentially extracted in 0.5 M and 1 M NaCl. After the 1.0-M salt extract, the remaining pellet was sonicated and digested with DNase. Ten micrograms of the 0.5-M and 1.0-M NaCl extracts and 100 μg of the DNase-digested extracts were separated by SDS-PAGE and immunoblotted for topo IIβ.

Adhesion of cells to FN increased the amount of topo IIβ bound to the nucleus after a 1.0-M NaCl extract.

Nuclei isolated from FN-adhered cells or cells in suspension were sequentially extracted in 0.5 M and 1 M NaCl. After the 1.0-M salt extract, the remaining pellet was sonicated and digested with DNase. Ten micrograms of the 0.5-M and 1.0-M NaCl extracts and 100 μg of the DNase-digested extracts were separated by SDS-PAGE and immunoblotted for topo IIβ.

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