Effect of platelet-type vWD mutations on adhesion to vWf under flow conditions.

Flow-based adhesion assays were performed as described in “Materials and methods.” (A) Cells were perfused at 1 dyne/cm², and cell tethering was quantitated at 1, 3, and 5 minutes after cells first entered the capillary tube. Ten fields were analyzed at each time point for each cell line. Data are the mean ± SEM from 3 or 4 experiments. (B) Rolling velocities were compared for all cell lines at a shear stress of 5, 20, and 40 dynes/cm². For each cell line, 5 randomly selected cells per field over 5 fields were analyzed for rolling velocity. Data are the mean ± SEM from 3 or 4 experiments. (Note: the rolling velocity of wild-type cells at 40 dynes/cm² was only analyzed in one experiment given that in most experiments insufficient cells remained adherent for quantitation purposes).