Fig. 2.
![Fig. 2. Reactivity of PA autoantibodies with KO variant GPIIb-IIIa. / (A) Reaction of mAbs (AP3 and OP-G2) and antiplatelet alloantibodies (anti–HPA-1a and anti–HPA-3a) with KO variant GPIIb-IIIa. (B) PA autoantibodies from 11 patients with chronic ITP, showing a marked decrease (greater than 50%) in the reactivity against KO variant compared with WT GPIIb-IIIa. Results are expressed as ΔOD: [OD value obtained from a test sample − (mean + 3 SD) OD value obtained from 5 control samples]. Open columns represent ΔOD value in antigen-capture ELISA using WT GPIIb-IIIa, and filled columns represent ΔOD value in antigen-capture ELISA using KO mutant. Data are representative of 2 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/6/10.1182_blood.v98.6.1819/7/h81811514002.jpeg?Expires=1735530397&Signature=jK2ZMZ0zmWLgzqaif0H56v1qK4YOksx6hLDzQCQGLUmtYPK8u3sGMk4fEZPVQ1BkH4n7c-MCGuSqOH3fJ41bzeBzEjVgCnb4JE1iOTuq6YtZVM6lTLKxHYd5bzbmuN~wNPEDMsS7a9bUaeH5mqKWlxrIDfSd8ZxyQRrc--W4vKd2ii28GThTxXFk-q7Vwsyimutsof3Zq6O5rF-6~wFBiDvBQVX~xA6LBuBdOanS~2L9Psyic90AwJF4kL6BG5eWL4Ps9hvpgCsg3fG~O1H-LdBdqVV4BcrGlRRhWFjax1LRapYAF9ffvI5jKjZVFniwdiQo4V3e6H4knHegnzNMLQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reactivity of PA autoantibodies with KO variant GPIIb-IIIa.
(A) Reaction of mAbs (AP3 and OP-G2) and antiplatelet alloantibodies (anti–HPA-1a and anti–HPA-3a) with KO variant GPIIb-IIIa. (B) PA autoantibodies from 11 patients with chronic ITP, showing a marked decrease (greater than 50%) in the reactivity against KO variant compared with WT GPIIb-IIIa. Results are expressed as ΔOD: [OD value obtained from a test sample − (mean + 3 SD) OD value obtained from 5 control samples]. Open columns represent ΔOD value in antigen-capture ELISA using WT GPIIb-IIIa, and filled columns represent ΔOD value in antigen-capture ELISA using KO mutant. Data are representative of 2 separate experiments.
