FACS sorting regions used for preparation of test cell populations from the muscle of chimeric mice and an example of analysis of hematopoietic engraftment by FACS-sorted cells.

(A) Ly-5.1⁺ cells and Ly-5.1⁻ cells were separated from mononuclear cells of the muscle of chimeric mice by FACS analysis with the use of R1 and R2 gates, respectively, for secondary-transplantation studies. Dot plots obtained in experiment 8 in Table 5 are shown. (B) Analysis of engraftment 2 months after secondary transplantation. Ly-5.1⁺ (R1) cells engrafted in the Ly-5.2 recipient. Ly-5.1⁻ (R2) cells transplanted into the Ly-5.1 recipient did not show hematopoietic engraftment by Ly-5.2 cells.