Fluorescein-tagged VEGF ODNs are taken up by various tumor cell lines in vitro.

Shown are the FITC images in the first column of treatments as indicated and the propidium iodide (PI) nuclear stain in the second column. Overlay images of ODN fluorescein signal exposed to AS-3, AS-3 mut1, and AS-3 mut2 (1 μM) are in the third column and show colocalization of the FITC and PI staining, indicating that the ODNs have entered the nuclei. Control was no treatment (no fluorescent AS-ODN; not shown).