Decorin increases macrophage adhesion.

(A) Cells (10 000) were cultured on plates precoated with BSA (■) or 10 μg/mL decorin (░) for only 30 and 60 minutes. After several washes, adhesion of macrophages was analyzed by crystal violet staining as indicated in “Materials and methods.” Each determination was made in triplicate, and the values represented correspond to the mean ± SD of 5 independent experiments. (B) Photographs of BMDMs attached to BSA-coated (control) or decorin-coated surfaces using a phase contrast microscope with an objective of × 40.