![Fig. 5. The adhesion of macrophages modulate their proliferation. / (A) Macrophages adhere preferably to fibronectin- or vitronectin-precoated surfaces. Cells (10 000) were cultured for 60 minutes on plates precoated with 10 μg/mL BSA (control), 10 μg/mL of the indicated components of the ECM, or 25 μg/mL P-OH-M, an inhibitor of cell adhesion. Macrophage adhesion was analyzed by crystal violet staining. Each determination was made in triplicate, and the values represented correspond to the mean ± SD of 3 independent experiments. (B) Adhesion modulates macrophage proliferation. Macrophages adhered to plates precoated with 10 μg/mL BSA, laminin or fibronectin, or 25 μg/mL P-OH-M were stimulated with the indicated concentrations of M-CSF and their proliferation was analyzed by [3H]-thymidine incorporation after 24 hours. Each point was made in triplicate, and the values represented correspond to the mean ± SD of 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/7/10.1182_blood.v98.7.2124/5/h81911594005.jpeg?Expires=1735556429&Signature=s1Qw3qqwGtvfED5ovPRaoxSRUsQls0szbD~M6I1YFgUjpt5DuhMSEEdr7vn5bnfFoyOly~6ndn3OoUrK68VJ-2eio-EmcL-b~iiA~Eev713~SskR7m3EZulZK6~gbHDmIsgjrVE8DgGsi8v0yQs7kPbzsyK1wgCQXbTGxrKGVjgysb5wnfSG0DxJY90JzWlNqkEwK3vlOUetR-~2nBU~uz9mgp5lgSsizemQMyRsZdzKkWBjNNh3FVBMET0NnhM6s14byeQJVLjk~s4flSyVxctUThqWF6QYZ4F2wthdsahYak6LojiO5BKWQYUtW8jKynyJyF2HluCmIiDbMa-LlA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The adhesion of macrophages modulate their proliferation.
(A) Macrophages adhere preferably to fibronectin- or vitronectin-precoated surfaces. Cells (10 000) were cultured for 60 minutes on plates precoated with 10 μg/mL BSA (control), 10 μg/mL of the indicated components of the ECM, or 25 μg/mL P-OH-M, an inhibitor of cell adhesion. Macrophage adhesion was analyzed by crystal violet staining. Each determination was made in triplicate, and the values represented correspond to the mean ± SD of 3 independent experiments. (B) Adhesion modulates macrophage proliferation. Macrophages adhered to plates precoated with 10 μg/mL BSA, laminin or fibronectin, or 25 μg/mL P-OH-M were stimulated with the indicated concentrations of M-CSF and their proliferation was analyzed by [3H]-thymidine incorporation after 24 hours. Each point was made in triplicate, and the values represented correspond to the mean ± SD of 2 independent experiments.
