After semiallogeneic BMT, donor T cells display a memory-activated phenotype in GCV-treated thymectomized recipients.

Experiments were performed on splenocytes recovered at day 78 after BMT and GCV administration. 84% ± 1% of mCD4⁺ cells express the hCD4 marker. Memory-activation markers were analyzed on mCD4⁺ hCD4⁺-gated T cells from grafted mice (n = 9) and compared to a pool of donor T cells before infusion (n = 3). Isotype-matched negative control is represented by a black histogram. For the semiallogeneic BMT group, cell frequencies are given as mean ± SEM.