Fig. 8.
Effect of Tat-dn-H-Ras and MEK inhibitor U0126 on eosinophil viability.
Blood eosinophils were incubated with no IL-5 (▧), 10 pM IL-5 (▪), 100 nM Tat-dn-H-Ras (■), 100 nM Tat-GFP (░), 10 μM U0126 (▩), or 50 μM PD98059 (▤) for up to 60 hours. Viability was determined by FITC-annexin V and propidium iodide labeling of the cells and subsequent flow cytometry. Accumulated data of 3 independent experiments are represented.