Fig. 2.
z-VAD.fmk inhibits caspase processing in IL-5–deprived peripheral blood eosinophils.
Cells were incubated with IL-5 (10−10 M), STS (10−5 M), or z-VAD.fmk (100 μM), harvested at the times indicated and analyzed for caspase-3 (A), -6 (B), -7 (C), -8 (D), and -9 (E) processing by immunoblotting. Eosinophils constitutively expressed caspase-9, which was activated during STS-induced and spontaneous apoptosis. IL-5 prevented the processing of caspase-9 observed during spontaneous apoptosis over the 24-hour culture period. z-VAD.fmk inhibited caspase activation in IL-5–deprived eosinophils. Results are representative of 3 separate experiments.
