Fig. 2.
Fig. 2. Phenotypic analysis of immature thymocytes, determined by analysis of CD3, CD4, and CD8 expression, in uninfected and HIV-infected cells. / Cells were isolated as described in “Materials and methods,” infected with a primary isolate HIV-1B-LAIp, and maintained in culture in the presence of IL-7 (5 ng/mL), TNF-α (1 ng/mL), IL-1 (10 ng/mL), and IL-6 (10 ng/mL). Immunostaining of surface antigens was analyzed by flow cytometry at the time of cell isolation (day 0) and after 11 days of culture with or without HIV-1 infection. Data are given as percentages of labeled cells as a mean of independent experiments carried out with thymuses from 7 different donors. ▪ indicates control; ■, HIV.

Phenotypic analysis of immature thymocytes, determined by analysis of CD3, CD4, and CD8 expression, in uninfected and HIV-infected cells.

Cells were isolated as described in “Materials and methods,” infected with a primary isolate HIV-1B-LAIp, and maintained in culture in the presence of IL-7 (5 ng/mL), TNF-α (1 ng/mL), IL-1 (10 ng/mL), and IL-6 (10 ng/mL). Immunostaining of surface antigens was analyzed by flow cytometry at the time of cell isolation (day 0) and after 11 days of culture with or without HIV-1 infection. Data are given as percentages of labeled cells as a mean of independent experiments carried out with thymuses from 7 different donors. ▪ indicates control; ■, HIV.

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