Flow cytometric analysis of human peripheral blood monocytes obtained after leukapheresis and countercurrent centrifugation before and after cell culture.

(A) Fresh, uncultured monocytes. (B) Purity of monocytes prepared by combined leukapheresis elutriation. (C) Monocytes cultured in SFM in GM-CSF for 20 to 24 hours, then analyzed by flow cytometry. (D) Macropinocytosis by immature DCs prepared by culture in SFM and GM-CSF over time in culture. Results are representative of 3 experiments.