Expression of RelB by activated DCs.

Nuclear cell extracts from 2 donors (donor 1 lanes 1-3, donor 2 lanes 4-6) were immunoblotted with anti-RelB antiserum 1319, as described in “Materials and methods.” Lanes 1 and 4, immature DCs (GM-CSF–treated); lanes 2 and 5, DCs activated with calcium ionophore; lanes 3 and 6, DCs activated with IFN-γ, TNF-α, and CD40L. Lane 7 represents the control for RelB expression. Antiactin was used to ensure similar levels of protein loading. Results are representative of a single experiment with 3 other experiments demonstrating similar results.