Fig. 6.
Fig. 6. CSA inhibits DC activation by calcium ionophore but not the inhibition of IL-12 production caused by calcium ionophore. / DCs were activated as indicated for 40 hours in the presence or absence of 1 μM CSA. DC1, immature DCs treated with IFN-γ, TNF-α, and rhCD40L. CI, immature DCs treated with 150 ng/mL calcium ionophore, as described in “Materials and methods.” CD83 and CD80 expression were measured by flow cytometry using FITC- labeled anti–human CD83 and CD80, as described in “Materials and methods.” Secretion of p70 IL-12 was measured 24 hours after the activation of DCs using ELISA. Results are those of a representative experiment; 3 other experiments demonstrated similar results.

CSA inhibits DC activation by calcium ionophore but not the inhibition of IL-12 production caused by calcium ionophore.

DCs were activated as indicated for 40 hours in the presence or absence of 1 μM CSA. DC1, immature DCs treated with IFN-γ, TNF-α, and rhCD40L. CI, immature DCs treated with 150 ng/mL calcium ionophore, as described in “Materials and methods.” CD83 and CD80 expression were measured by flow cytometry using FITC- labeled anti–human CD83 and CD80, as described in “Materials and methods.” Secretion of p70 IL-12 was measured 24 hours after the activation of DCs using ELISA. Results are those of a representative experiment; 3 other experiments demonstrated similar results.

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