An ALCAM-transfected endothelial cell line supports hematopoiesis.

(A) EOMA was transfected with full-length ALCAM cDNA or vector alone. E11-derived yolk sac endothelial cell line, YSCL-72, and established ALCAM-transfected cell lines, GM2 and GM10, were examined for their expression of ALCAM by FACS. (B) ALCAM⁺ or ALCAM⁻ bone marrow mononuclear cells were cultured with vector-transfected stromal cells or ALCAM-transfected stromal cells (GM2). After 4 or 5 hours of incubation, cells were washed several times, and the remaining cells were analyzed for the expression of CD45; *P < .05. (C) A total of 1000 fetal liver–derived stem cells (Lin⁻Sca-1⁺c-Kit⁺) were plated onto each stromal cell line and cultured for 6 days without cytokines. Nonadherent cells were harvested, and the cell number was scored; *P < .05.