Examination of ability to support yolk sac–derived hematopoiesis in an ALCAM-transfected endothelial cell line.

(A) E10.5-derived yolk sac cells were stained with anti-CD34 antibody, anti–c-Kit antibody, and anti-ALCAM antibody. CD34⁺c-Kit⁺ cells were subdivided on the basis of the expression of ALCAM. (B) A total of 1000 CD34⁺c-Kit⁺ALCAM⁻ or CD34⁺c-Kit⁺ALCAM⁺ cells were cultured with GM2 cells (ALCAM-transfected cell line) or vector-transfected cells for 7 days. The number of nonadherent cell was scored (Bi), and expression of c-Kit was analyzed by FACS (Bii). *P < .05.