Cytoplasmic Mpl supports proliferation of murine marrow cells indistinguishable from its membrane-tethered counterpart.

Murine bone marrow cells transduced with retroviral vectors encoding either membrane-targeted Mpl (closed circles) or cytoplasmic Mpl (open circles) were cultured in suspension in the presence or absence of AP20187 (100 nM). Cell growth failed to occur in the absence of AP20187 (data not shown). Results depict a single representative experiment from among 4 independent transductions.