Reduced homing capacity of murine FL and MPB cells compared to BM.
Lethally irradiated mice were transplanted with about 107to 108 PKH26-labeled BM, FL, or MPB cells. Three hours later, PKH26+ cells that had homed to the BM (■) or spleen (▪) were quantitated by flow cytometry and isolated by FACS for CFC assays. Shown are the mean ± SEM percent recovery of donor-derived cells (left) and CFCs (right) per organ relative to numbers injected. See “Materials and methods” for details of recovery calculations. Pooled data are from 3 BM, 5 FL, and 4 MPB cell experiments (homing was assayed in one mouse per experiment). Differences between BM and either FL or MPB cell and CFC homing are significant (P < .05) in all cases except for BM versus MPB-derived CFCs homing to BM where the latter value is derived from a single measurement. Data for BM cells are reproduced from Szilvassy and colleagues.17
