Fig. 1.
Fig. 1. Genomic structure of the transgenes. / (A) Genomic structure of the floxed Piga gene (L) before(loxPiga) and after (ΔPiga) Cre-mediated loxP recombination. Cre-mediatedloxPiga gene recombination deletes 662 base pairs ofPiga exon 2 and 1.6 kilobases (kb) of the adjoining intron 2, which completely abrogates the function of PIGA.8 (B) Map of the GATA1-cre transgene in G mice. IE refers to the erythroid GATA-1 promoter, 5′HS4, a 5′ element of the chicken β-globin locus that functions as an insulator (gray oval).23 Exons are boxed and numbered; loxP sites are shown as triangles; restriction endonuclease sites are indicated.

Genomic structure of the transgenes.

(A) Genomic structure of the floxed Piga gene (L) before(loxPiga) and after (ΔPiga) Cre-mediated loxP recombination. Cre-mediatedloxPiga gene recombination deletes 662 base pairs ofPiga exon 2 and 1.6 kilobases (kb) of the adjoining intron 2, which completely abrogates the function of PIGA.8 (B) Map of the GATA1-cre transgene in G mice. IE refers to the erythroid GATA-1 promoter, 5′HS4, a 5′ element of the chicken β-globin locus that functions as an insulator (gray oval).23 Exons are boxed and numbered; loxP sites are shown as triangles; restriction endonuclease sites are indicated.

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