Loss of CD24 expression during erythroid differentiation.

The loss of GPI-linked proteins on red cell precursors from GL mice occurs with the expression of Ter119. Single-cell suspensions of fetal liver cells from E13.5 fetuses and bone marrow cells (BM) from adult mice were analyzed for the expression of Ter119 and CD24. Note that while in E^+/−L^+/− mice a distinct proportion of Ter119^high and Ter119^low cells are deficient in CD24, in GL mice a significant proportion of CD24-deficient cells are found only in the Ter119^high cell population. L^+/− indicates control mouse. E^+/−L^+/− mice are offspring from theEIIa-cre × L breeding8 and are mosaic for a nonfunctional PIGA protein. Numbers indicate the percentage of cells in the left upper and left lower quadrant (cells deficient in CD24). Vertical marker shown delineates CD24⁻ from CD24⁺ Ter119^low cells.