Expression of CD24 on individual BFU-Es from GL mice.

BFU-E colonies grown in methylcellulose from bone marrow cells of GL mice were individually picked and analyzed for the expression of CD24. Visual inspection, high CD71 expression, and the lack of CD11b were used to distinguish BFU-Es from mast cell and myeloid colonies. Dead cells and debris were excluded by their light scatter characteristics (the gates used are shown on the left). Numbers indicate the percentage of cells with normal decreased expression of CD24.