EMSA of primitive AML cells.

Quiescent CD34⁺38⁻AML cells demonstrate NF-κB binding activity. (A) Representative example (specimen 1 in Table 1) of an EMSA shows NF-κB binding for AML CD34⁺/CD38⁻/CD123⁺ cells. As shown for Figure 1B, binding is competed by wild-type, but not mutant oligonucleotide, and both p50 and p65 subunits are detected. (B) Representative example of AML cells gated for CD34⁺/38⁻ (left panel) and labeled by the SID method of cell-cycle analysis (right panel). An identical pattern of NF-κB binding was observed for 3 of 3 CD34⁺/CD38⁻/CD123⁺ AML specimens.