Nucleotide sequence of s-SHIP cDNA and the predicted amino acid sequence of its major ORF.

The first 784 nucleotides of the SHIP cDNA are provided for comparison above the bold arrow. s-SHIP cDNA sequence is indicated below the bold arrow, starting with the numeral 1 and continuing through nucleotide 2946. Remaining s-SHIP cDNA sequence (nucleotides 2947-4125) is not shown because of size constraints, but it is identical to nucleotides 3687 to 4865 of the SHIP cDNA (data not shown). The number in parentheses (785) at nucleotide 45 of the s-SHIP cDNA indicates the nucleotide in the SHIP cDNA, where identity between the s-SHIP and SHIP cDNAs begins. ATG initiator codons for the SHIP and s-SHIP major ORFs are indicated in bold and underlined, and the termination codon shared by both isoforms is indicated by an asterisk. SH2 (dotted box), SSR (solid box), and inositol 5′-phosphatase (dashed box) sequences are shown. The nucleotide sequence that encodes the Δ183 nucleotide deletion is underlined. NPXY and proline-rich motifs are shaded black and gray, respectively.