Organization of the first exon of s-SHIP.

(A) Genomic sequence of the first exon of s-SHIP and adjacent intronic sequence. Uppercase letters represent the nucleotides in the first exon of s-SHIP, and lowercase letters represent the intronic sequence immediately flanking this exon. The 44 nucleotides enclosed in the shaded box indicate the SSR, and the 88 nucleotides in the clear box represent those found in both the s-SHIP and the 145-kd SHIP cDNAs. Numbers 785 and 872 indicate the position of each corresponding nucleotide in the murine 145-kd SHIP cDNA sequence (GenBank accession number U52044). (B) Schematic representation of the orientation of the predicted promoter regions and first exons of SHIP and s-SHIP relative to each other in the SHIP locus. Arrows indicate the transcriptional start points. The s-SHIP first exon consists of the 44-nucleotide SSR and the adjacent 88-nucleotide SHIP exon 6.39 Nucleotide numbering refers to the 145-kd SHIP cDNA sequence (accession number U52044).