Fig. 5.
Fig. 5. HSCs express s-SHIP mRNA. / HSCs and lineage-committed cells were sorted from ABM or day 14.5 FL and analyzed by nested RT-PCR assay for the expression of s-SHIP, SHIP, and β-actin mRNA. In both ABM and FL, s-SHIP mRNA was detected in HSCs (Sca-1+c-kit+Lin−) but not in the myeloid (Gr-1+/Mac-1+), B-lymphoid (B220+), or erythroid (Ter119+) lineages. Hepa 1-6 cells were sorted as the negative control for both s-SHIP and SHIP mRNA expression, and ES-TL1 and WEHI-231 cells were sorted as positive controls for s-SHIP and SHIP mRNA expression, respectively.

HSCs express s-SHIP mRNA.

HSCs and lineage-committed cells were sorted from ABM or day 14.5 FL and analyzed by nested RT-PCR assay for the expression of s-SHIP, SHIP, and β-actin mRNA. In both ABM and FL, s-SHIP mRNA was detected in HSCs (Sca-1+c-kit+Lin−) but not in the myeloid (Gr-1+/Mac-1+), B-lymphoid (B220+), or erythroid (Ter119+) lineages. Hepa 1-6 cells were sorted as the negative control for both s-SHIP and SHIP mRNA expression, and ES-TL1 and WEHI-231 cells were sorted as positive controls for s-SHIP and SHIP mRNA expression, respectively.

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