Organization of the first exon of SIP-110, the human homolog of s-SHIP.

(A) Genomic sequence of the SIP-110 first exon and adjacent intronic sequence as compiled from the Celera Human Genome-Unassembled Fragments database (Celera accession numbers GA_x4N24A5J0RM:1..262 and GA_x4N24A6F9UD:1..508). Uppercase letters represent the nucleotides in the SIP-110 first exon, and lowercase letters represent the intronic sequence immediately flanking this exon. The 42 nucleotides enclosed in the shaded box indicate the SSR-like region, and the 88 nucleotides in the clear box represent those nucleotides found in both the SIP-110 and the human 145-kd SHIP cDNAs. Based on the genomic fragment sequences, position 1 is listed as a “T” rather than as the “C” assigned in the SIP-110 cDNA (GenBank accession number U50040). Numbers 787 and 874 indicate the position of the corresponding nucleotide in the human 145-kd SHIP cDNA sequence (GenBank accession number U50041). (B) Clustal W alignment33 of the first exon sequences of human SIP-110 and murine s-SHIP. Matching nucleotides are shaded gray. Overall, the first exons of SIP-110 and s-SHIP show 82% nucleotide identity.