PD98059 and SB203580 differentially regulate the phenotypic changes that take place during TNF-α–induced maturation of MDDC.

Immature MDDC were either not treated (control) or treated with TNF-α in the absence of inhibitors (TNF-α) or in presence of PD98059 (PD and TNF-α) or SB203580 (SB and TNF-α). After 24 hours (A) or 48 hours (B,C), cell-surface expression of CD83, CD49d, CD86, MHC class II, CD11c, and CD40 was determined by indirect immunofluorescence and fluorescence of a negative control antibody (X63). Flow cytometry profiles are shown in panel A, and data are given as an expression index (percentage of marker-positive cells multiplied by their MFI) in panels B and C. Each assessment was done at least 4 times and representative results are shown. The results shown in panels A and B and in panel C were obtained with samples from 2 different donors.