Biodistributions of radioactivity in blood, tumor, and normal organs of Ramos xenograft-bearing athymic mice injected with either directly labeled 1F5 antibody or pretargeted 1F5-SA conjugate.

Mice were injected either with 1.4 nmol conventional trace-labeled¹²⁵I-1F5 (A) or 1.4 nmol ¹²⁵1F5-SA (B) followed 24 hours later by 5.8 nmol CA and 3 hours after that by 1.2 nmol¹¹¹In-DOTA-biotin (B). Groups of 5 mice were euthanized 2, 12, 24, 48, 96, and 144 hours after injection of the radiolabeled species and the radioactivity in blood, tumor, and normal organs was quantified by gamma counting and expressed as the percent of the injected dose of radioactivity present per gram of tissue. Tumor (●, heavy solid line), blood (○, dashed line), kidney (▪), liver (▴), lung (×), muscle (*), small intestine (♦).