Treatment with Mpl ligand does not impair the p53 response to carboplatin and radiation.

Bone marrow cells of C57Bl/6J mice (1 mouse per group) were harvested and solubilized at the indicated intervals after treatment with 80 mg/kg carboplatin (administered intravenously) and 7.5 Gy TBI followed immediately by PEG-rmMGDF or carrier. Samples were assessed by immunoblotting for expression of p53, Bax, and p21^Cip1. Immunoblotting of actin on the same immunoblot is shown for comparison of protein loading per lane. Unt: untreated (bone marrow cells from C57Bl/6J mice that received no carboplatin/TBI).