Expression of scinderin and gelsolin in cell lines.

MEG-01 cells were transfected with either pcDNA3 vectors (control) or the vectors carrying full-length scinderin (Sc) cDNA (pcDNA3-Sc) inserts, as indicated in “Materials and methods.” (A) Coomassie blue-stained SDS-PAGE gel shows Sc protein bands (Sc) for 2 Sc-expressing clones. The band is not seen in the gel corresponding to cells transfected with the pcDNA3 vector (left lane). (B) Western blot performed with a polyclonal antibody raised against recombinant Sc. Lanes from left to right: cell lines K562, HEL, NS-MEG, MEG-01 (wild-type) cells, cells transfected with pcDNA3, 4 Sc-expressing clones, and chromaffin cells (positive control). Immunostaining with Sc antibody of cells transfected with pcDNA3-Sc (C) and pcDNA3 (D). (E) Western blot with a monoclonal gelsolin antibody (top) and levels of gelsolin expression (bottom) in MEG-01 cells transfected with either pcDNA3 or pcDNA3-Sc. Tubulin levels detected by a polyclonal antibody were used as control for gel loading. Bars represent mean ± SEM from 4 different experiments (*P < .001).