Fig. 7.
Fig. 7. Effect of scinderin expression on cellular levels and activity of different transduction and transcription factors. / Extracts were prepared from cells transfected with either pcDNA3 or pcDNA3-Sc and cultured for 14 days. SDS-PAGE of the extracts was performed in quadruplicate and was followed by immunoblotting. Western blots with the corresponding antibodies are shown for Ras and Cdc42 (A); Rac2 and RhoA (B); PAK, JNK1, and JNK2 (C); c-jun and c-fos (D); and PAK activity using histone H4 as substrate (E). Tubulin was used as gel-loading control and to express results as a ratio between each protein and tubulin after imaging bands from the autoradiographs. (F) Time course of Rac2 and gelsolin expression in cells transfected with pcDNA3-Sc. Bars and circles represent mean ± SEM for those experiments (**P < .05; *P < .01; ΔP < .001).

Effect of scinderin expression on cellular levels and activity of different transduction and transcription factors.

Extracts were prepared from cells transfected with either pcDNA3 or pcDNA3-Sc and cultured for 14 days. SDS-PAGE of the extracts was performed in quadruplicate and was followed by immunoblotting. Western blots with the corresponding antibodies are shown for Ras and Cdc42 (A); Rac2 and RhoA (B); PAK, JNK1, and JNK2 (C); c-jun and c-fos (D); and PAK activity using histone H4 as substrate (E). Tubulin was used as gel-loading control and to express results as a ratio between each protein and tubulin after imaging bands from the autoradiographs. (F) Time course of Rac2 and gelsolin expression in cells transfected with pcDNA3-Sc. Bars and circles represent mean ± SEM for those experiments (**P < .05; *P < .01; ΔP < .001).

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