Expression of scinderin inhibits tumor growth in nude mice.
Balb/c nude mice were injected with cells cultured for 14 days after transfection with either vector (pcDNA3) alone or the same vector carrying a full-length insert of Scinderin cDNA (pcDNA3-Sc). (A) Tumor volumes after subcutaneous injection (inj) with 107 cells in 100 μL saline for each condition. Open and closed circles represent mean ± SEM from 2 groups of 9 mice each (*P < .05). †All pcDNA3-transfected mice were killed at 3 weeks after injection in accordance with institutional animal care policies. (B) Hematoxylin-eosin staining of a section from a large tumor produced by pcDNA3-transfected cells. Histology of 8 remaining tumors in this group was similar, and so was that of tumors formed by wild-type cells (data not shown). (C) Similar staining of a section from 1 of the 2 small tumors produced by pcDNA3-Sc–transfected cells showing apoptotic nuclei surrounded by numerous platelet-like particles. The second small tumor in this group had a similar histology.
