Fig. 3.
![Fig. 3. Cell proliferation over time with and without IL-10 in the vaccination models. / Proliferation assays were performed in the presence and absence of antigen, cytokines (100 U/mL of IL-2 and IL-15, respectively) or PMA–ionomycin (PMA, 25 ng/mL; ionomycin, 0.5 μM) using splenocytes (1 × 106 cells/well) harvested from mice 14, 21, and 28 days after DC vaccination in each group depicted in Figure 2. After 48-hour culture, 0.037 MBq (1 μCi) [3H]-thymidine was added, and cells were harvested 16 hours later. Statistically significant differences relative to DC-vaccinated mice that did not receive IL-10 are indicated. Control mice were immunized with DCs in the absence of peptide. Each bar represents the mean of 3 mice evaluated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/7/10.1182_blood.v98.7.2143/5/h81911575003.jpeg?Expires=1735555245&Signature=EqkCZlyYStEd4Tuy1JPXua3d-FKaa1OhUV0iL86lmxziufYz1RzwqinuwMp1e4W4Yt5PTXEVAM2wZJjpxV1axJDV1T1EVLoEkKoHu3fyAP4e8LhSYn88XqA0CN1IPPaiDmfUVLrF1Sok6t4FSnyJbjkAZhpgpE91kwGWBLmB~IBpy0h2E1GVbotjvWLI0XFWPczhWoykoy6uKKml7mECg55AZo89woHaEGooO8MxI8Zn82y9rWKHz2iteje2yD3CU~lw8M~pDUpJSwuz5r9RtDLAzoygHZcZAr~cqMASKTN7L6nb4AqBmIBqTHPWa9PU52BicFkLDg54wauc8OBP9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell proliferation over time with and without IL-10 in the vaccination models.
Proliferation assays were performed in the presence and absence of antigen, cytokines (100 U/mL of IL-2 and IL-15, respectively) or PMA–ionomycin (PMA, 25 ng/mL; ionomycin, 0.5 μM) using splenocytes (1 × 106 cells/well) harvested from mice 14, 21, and 28 days after DC vaccination in each group depicted in Figure 2. After 48-hour culture, 0.037 MBq (1 μCi) [3H]-thymidine was added, and cells were harvested 16 hours later. Statistically significant differences relative to DC-vaccinated mice that did not receive IL-10 are indicated. Control mice were immunized with DCs in the absence of peptide. Each bar represents the mean of 3 mice evaluated.
