Fig. 7.
Fig. 7. Characterization of naive and activated OVA-specific OT-1 T cells from spleen cells of OT-1 mice. / (A) Isolated CD8+ T cells from spleen cells of OT-1 mice were analyzed by 2-color flow cytometric analysis using anti-CD8 and anti-Vβ 5.1 and 5.2 mAbs. (B) After 36 hours culture with 1 × 10−6 M OVA peptide-pulsed syngeneic C57BL/b spleen cells, these T cells expressed an activated phenotype—ie, they expressed CD25, CD69, and CD44 antigen. (C) They also produced IFN-γ but not IL-2 and IL-4.

Characterization of naive and activated OVA-specific OT-1 T cells from spleen cells of OT-1 mice.

(A) Isolated CD8+ T cells from spleen cells of OT-1 mice were analyzed by 2-color flow cytometric analysis using anti-CD8 and anti-Vβ 5.1 and 5.2 mAbs. (B) After 36 hours culture with 1 × 10−6 M OVA peptide-pulsed syngeneic C57BL/b spleen cells, these T cells expressed an activated phenotype—ie, they expressed CD25, CD69, and CD44 antigen. (C) They also produced IFN-γ but not IL-2 and IL-4.

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