Normal and abnormal patterns of hybridization with both strategies.

(A) Hybridization of the V_H and C_H probes to the correct location at band 14q32 on normal human male metaphases. (B) Normal pattern for the V_HC_H probes with 2 pairs of closely associated signals. Note the intense blue fluorescence of the cytoplasmic light chain. (C) Cellular signal pattern indicative of a 14q32 translocation. The segregation of a pair of signals (break-apart) in a large percentage of cells allows for the identification of a patient with an IgH translocation. (D) Hybridization of the 14q32 and 11q13 probes to the correct locations on chromosomes 14 and 11, respectively, on normal human male metaphases. (E) Normal pattern for the 14q32/11q13 fusion strategy. There are no fusion signals observed, and there are 2 pairs of nonassociated green (14q32) and red (11q13) signals. (F) Abnormal comigration (fusion) of signals indicative of a t(11;14)(q13;q32). Because of the small signal size arising from the V_Hportion of the IgH probe (owing to the V/D/J recombination and DNA excision), the pattern most frequently observed is presumed to be that of fusions in the der14 and less commonly in the der11.