Identification of blood DCs for quantification and expression of HIV-1 receptor and coreceptor molecules.

DCs were identified by FACS analysis on the basis of their lack of labeling for the mononuclear cell markers CD3, CD14, CD16, and CD19 but positive staining for HLA-DR. The myDCs were differentiated from the pcDCs by their expression of CD11c (A). (B) Histograms show expression of CD4, CXCR4, and CCR5 on myDCs and pcDCs. In each plot, the solid curve depicts labeling with an isotype control antibody and the open curve shows labeling with a specific antireceptor antibody.