Expression of TRAIL in uninfected and HHV-7–infected SupT1 cells, evaluated by immunoblotting analysis of cell lysates.

(A) Virus growth was detected by indirect immunofluorescence using the HHV-7–specific 5E mAb. Representative photographs taken 8 days after infection are shown. (left panels) HHV-7–specific immunofluorescence. (right panels) DAPI staining of the nuclei of the same field. Original magnification is × 250. (B) Equivalent amounts of protein lysates obtained from HHV-7–infected (inf) and uninfected (uninf) SupT1 cells were analyzed by Western blot with anti-TRAIL mAb. Equal loading of protein in each lane was confirmed by staining with the antibody to β-actin. Molecular size markers are indicated on the right in kilodaltons. Relative intensities of the bands were densitometrically quantified and expressed in arbitrary units (AU). Results are representative of 5 independent experiments performed.