Surface TRAIL-R1 and TRAIL-R2 expression analyzed in combination with surface CD4 in SupT1 either uninfected or infected with HHV-7.

(A) At 8 days after infection, relative surface CD4 expression was detected by PE-Cy5 fluorescence intensity (x-axis), whereas TRAIL-R1 and TRAIL-R2 expression was detected by PE fluorescence intensity (y-axis). Representative negative controls, composed of cells stained with irrelevant isotype-matched Ab (Irr Abs) are shown in the top panels. (B) Evaluation of surface CD4 expression in HHV-7–infected cultures after 20 hours of treatment with rTRAIL or rHis6-tag control (cont) peptide. X-axis, relative surface CD4 expression detected by PE-Cy5 fluorescence intensity. Y-axis, relative cell number. Data shown are from a single experiment representative of 5 independent experiments with similar results.