Fig. 6.
A rise in DC [Ca++]i follows interaction with CD8+ but not CD4+ T cells.
Enriched CD8+ (A) or CD4+ (B) alloreactive T cells loaded with FURA 2-am were added to FURA 2-am–loaded DCs and the oscillations in [Ca++]i were monitored at the single cell level. Pictures were taken at intervals of 10 minutes. Panels C and D show the mean of 15 Ca++ responses induced in DCs by CD8+ alloreactive T cells (C) and in CD4+alloreactive T cells by DCs (D). Results are expressed as ratio of the light emitted at 510 nm after excitation at 334 nm or 380 nm. One representative experiment of 3 is shown.